It is one of the most frequent causes of pain, loss of function and disability in adults affecting to up 40% of those aged over 65 (Arden and Nevitt 2006 Bijlsma et al. Osteoarthritis is a degenerative joint disease characterized by tensile stiffness, degeneration and progressive loss of articular cartilage. Thus, increasing glycine in the diet may well be a strategy for helping cartilage regeneration by enhancing collagen synthesis, which could contribute to the treatment and prevention of osteoarthritis. Since the large effects produced by proline and lysine are within the physiological range, while the effect of glycine corresponds to a much higher range, these results demonstrated a severe glycine deficiency for collagen synthesis. Increase of glycine as of 1.0 mM exceeds these effects and this increase continues more persistently by 60–75%. Increase in concentrations of proline and lysine in the culture medium enhances the synthesis of type II collagen at low concentrations, but these effects decay before 1.0 mM. Type II collagen was measured in the monolayer, every 48 h for 15 days by ELISA. Cell growth and viability were assayed by trypan blue dye exclusion method. Chondrocytes were characterized by type II collagen immunocytochemistry of confluence monolayer cultures. Bovine articular chondrocytes were cultured under a wide range of different concentrations of glycine, proline and lysine. In this work we studied the effect of these amino acids on type II collagen synthesis. Other authors have shown that proline is conditionally essential. Previous works of our group have shown that glycine is an essential amino acid, which must be present in the diet in large amounts to satisfy the demands for collagen synthesis. This requires large amounts of glycine, proline and lysine. Collagen synthesis is severely diminished in osteoarthritis thus, enhancing it may help the regeneration of cartilage.
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